/
/
Production and Purification of Non Replicative Canine Adenovirus Type 2 Derived Vectors
È necessario avere un abbonamento a JoVE per visualizzare questo. Accedi o inizia la tua prova gratuita.
Production and Purification of Non Replicative Canine Adenovirus Type 2 Derived Vectors
6,957 Views
•
14:55 min
•
December 03, 2013
DOI:
Summary
Automatically generated
These past 15 years, canine adenovirus type 2 (CAV2)-derived vectors have proven their efficiency to transduce cells in vitro and in vivo and are widely used for vaccination and gene therapy. Here, we describe a procedure to construct, produce and purify CAV2 vectors, giving rise to high-titer viral suspensions.
Read Article
Cite this Article
Szelechowski, M., Bergeron, C., Gonzalez-Dunia, D., Klonjkowski, B. Production and Purification of Non Replicative Canine Adenovirus Type 2 Derived Vectors. J. Vis. Exp. (82), e50833, doi:10.3791/50833 (2013).
Copy