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In vitro Coculture Assay to Assess Pathogen Induced Neutrophil Trans-epithelial Migration
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JoVE Journal İmmünoloji ve Enfeksiyon
In vitro Coculture Assay to Assess Pathogen Induced Neutrophil Trans-epithelial Migration

In vitro Coculture Assay to Assess Pathogen Induced Neutrophil Trans-epithelial Migration

17,240 Views

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14:14 min

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January 06, 2014

DOI:

10.3791/50823-v

DOI:
10.3791/50823-v

•

14:14 min
January 06, 2014

•

4 Views
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1Pediatri Anabilim Dalı,Harvard Tıp Fakültesi, 2Department of Pediatric Gastroenterology,MGH for Children, 3Mucosal Immunology and Biology Research Center,Massachusetts General Hospital

Özet

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Neutrophil trans-epithelial migration in response to mucosal bacterial infection contributes to epithelial injury and clinical disease. An in vitro model has been developed that combines pathogen, human neutrophils, and polarized human epithelial cell layers grown on transwell filters to facilitate investigations towards unraveling the molecular mechanisms orchestrating this phenomenon.

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Kusek, M. E., Pazos, M. A., Pirzai, W., Hurley, B. P. In vitro Coculture Assay to Assess Pathogen Induced Neutrophil Trans-epithelial Migration. J. Vis. Exp. (83), e50823, doi:10.3791/50823 (2014).

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