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In vivo méthode d'imagerie à distinguer inflammation aiguë et chronique
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Immunology and Infection
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JoVE Journal Immunology and Infection
In vivo Imaging Method to Distinguish Acute and Chronic Inflammation
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In vivo méthode d'imagerie à distinguer inflammation aiguë et chronique

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19,914 Views

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07:10 min

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August 16, 2013

DOI:

10.3791/50690-v

DOI:
10.3791/50690-v

•

07:10 min
August 16, 2013

•

3 Views
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1Lurie Family Imaging Center, Dana-Farber Cancer Institute,Harvard Medical School, 2Division of Pediatric Hematology/Oncology/Stem Cell Transplantation,Columbia University Medical Center

Summary

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Nous décrivons une méthode d'imagerie non-invasive permettant de distinguer les stades inflammatoires. Administration systémique de luminol révèle des zones d'inflammation aiguë dépendent activité MPO dans les neutrophiles. En revanche, l'injection de lucigénine permet de visualiser l'inflammation chronique dépend de l'activité Phox dans les macrophages.

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InflammationAcute InflammationChronic InflammationNeutrophilsMacrophagesBioluminescence ImagingLuminolLucigeninMyeloperoxidase (MPO)Phagocyte NADPH Oxidase (Phox)Non-invasive ImagingPathogen Invasion

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Cite this Article

Tseng, J., Kung, A. L. In vivo Imaging Method to Distinguish Acute and Chronic Inflammation. J. Vis. Exp. (78), e50690, doi:10.3791/50690 (2013).

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