Dergi
/
İmmünoloji ve Enfeksiyon
/
In vivo Imaging Method to Distinguish Acute and Chronic Inflammation
JoVE Journal
İmmünoloji ve Enfeksiyon
Bu içeriği görüntülemek için JoVE aboneliği gereklidir.  Oturum açın veya ücretsiz deneme sürümünü başlatın.
JoVE Journal İmmünoloji ve Enfeksiyon
In vivo Imaging Method to Distinguish Acute and Chronic Inflammation

In vivo Imaging Method to Distinguish Acute and Chronic Inflammation

DOI:
10.3791/50690-v

•

07:10 min

•

August 16, 2013

•

,
1Lurie Family Imaging Center, Dana-Farber Cancer Institute,Harvard Tıp Fakültesi, 2Division of Pediatric Hematology/Oncology/Stem Cell Transplantation,Columbia University Medical Center

Bölümler

  • 00:05Başlık
  • 01:55Subcutaneous PMA Inflammation Model
  • 02:58Bioluminescence Imaging of Inflammation
  • 05:11Results: Longitudinal Bioluminescence Imaging of Inflammation
  • 06:39Conclusion

Özet

Otomatik Çeviri

Otomatik Çeviri

We describe a non-invasive imaging method for distinguishing inflammatory stages. Systemic delivery of luminol reveals areas of acute inflammation dependent upon MPO activity in neutrophils. In contrast, injection of lucigenin allows for visualization of chronic inflammation dependent upon Phox activity in macrophages.

Etiketler

InflammationAcute InflammationChronic InflammationNeutrophilsMacrophagesBioluminescence ImagingLuminolLucigeninMyeloperoxidase (MPO)Phagocyte NADPH Oxidase (Phox)Non-invasive ImagingPathogen Invasion

Article

Embed

ÇALMA LİSTESİNE EKLE

Usage Statistics

İlgili Videolar

Non-invasive Imaging of Leukocyte Homing and Migration in vivo

Using Eggs from Schistosoma mansoni as an In vivo Model of Helminth-induced Lung Inflammation

Bioluminescent Bacterial Imaging In Vivo

A Mouse Model for Pathogen-induced Chronic Inflammation at Local and Systemic Sites

An Optimized Method for Isolating and Expanding Invariant Natural Killer T Cells from Mouse Spleen

A Non-invasive and Technically Non-intensive Method for Induction and Phenotyping of Experimental Bacterial Pneumonia in Mice

Non-invasive In Vivo Fluorescence Optical Imaging of Inflammatory MMP Activity Using an Activatable Fluorescent Imaging Agent

Murine Lymphocyte Labeling by 64Cu-Antibody Receptor Targeting for In Vivo Cell Trafficking by PET/CT

Come to the Light Side: In Vivo Monitoring of Pseudomonas aeruginosa Biofilm Infections in Chronic Wounds in a Diabetic Hairless Murine Model

An Efficient and Simple Method to Establish NK and T Cell Lines from Patients with Chronic Active Epstein-Barr Virus Infection

Read Article