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Een<em> In-vitro</em> Voorbereiding van Geïsoleerde enterische neuronen en Glia uit de myenterische Plexus van de Adult Mouse
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Neuroscience
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JoVE Journal Neuroscience
An In-vitro Preparation of Isolated Enteric Neurons and Glia from the Myenteric Plexus of the Adult Mouse
Please note that all translations are automatically generated. Click here for the English version.

Een<em> In-vitro</em> Voorbereiding van Geïsoleerde enterische neuronen en Glia uit de myenterische Plexus van de Adult Mouse

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26,147 Views

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10:34 min

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August 07, 2013

DOI:

10.3791/50688-v

DOI:
10.3791/50688-v

•

10:34 min
August 07, 2013

•

18 Views
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1Pharmacology and Toxicology,Virginia Commonwealth University, 2Physiology and Biophyics,Virginia Commonwealth University

Summary

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We demonstrate a cell culture protocol for the direct study of neuronal and glial components of the enteric nervous system. A neuron/glia mixed culture on coverslips is prepared from the myenteric plexus of adult mouse providing the ability to examine individual neuron and glia function by electrophysiology, immunohistochemical, etc.

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Smith, T. H., Ngwainmbi, J., Grider, J. R., Dewey, W. L., Akbarali, H. I. An In-vitro Preparation of Isolated Enteric Neurons and Glia from the Myenteric Plexus of the Adult Mouse. J. Vis. Exp. (78), e50688, doi:10.3791/50688 (2013).

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