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An In-vitro Preparation of Isolated Enteric Neurons and Glia from the Myenteric Plexus of the Adult Mouse
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JoVE Journal Neurowissenschaften
An In-vitro Preparation of Isolated Enteric Neurons and Glia from the Myenteric Plexus of the Adult Mouse

An In-vitro Preparation of Isolated Enteric Neurons and Glia from the Myenteric Plexus of the Adult Mouse

DOI:
10.3791/50688-v

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10:34 min

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August 07, 2013

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, , , ,
1Pharmacology and Toxicology,Virginia Commonwealth University, 2Physiology and Biophyics,Virginia Commonwealth University

Kapitel

  • 00:05Titel
  • 02:04Longitudinal Muscle/Myenteric Plexus Harvesting
  • 04:42LMMP Digestion
  • 07:18Results: An in vitro Preparation of Isolated Enteric Neurons and Glia from the Myenteric Plexus of the Adult Mouse
  • 09:16Conclusion

Summary

Automatische Übersetzung

Automatische Übersetzung

We demonstrate a cell culture protocol for the direct study of neuronal and glial components of the enteric nervous system. A neuron/glia mixed culture on coverslips is prepared from the myenteric plexus of adult mouse providing the ability to examine individual neuron and glia function by electrophysiology, immunohistochemical, etc.

Tags

Enteric Nervous SystemMyenteric PlexusIn Vitro PreparationIsolated NeuronsEnteric GliaMouse IleumColonEnzymatic DigestionNeurite OutgrowthAction PotentialsGFAP StainingImmunohistochemistryElectrophysiologyCalcium ImagingSingle-cell PCRGenetically Modified Animals

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