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An In-vitro Preparation of Isolated Enteric Neurons and Glia from the Myenteric Plexus of the Adult Mouse
JoVE 杂志
神经科学
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JoVE 杂志 神经科学
An In-vitro Preparation of Isolated Enteric Neurons and Glia from the Myenteric Plexus of the Adult Mouse

An In-vitro Preparation of Isolated Enteric Neurons and Glia from the Myenteric Plexus of the Adult Mouse

DOI:
10.3791/50688-v

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10:34 min

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August 07, 2013

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, , , ,
1Pharmacology and Toxicology,Virginia Commonwealth University, 2Physiology and Biophyics,Virginia Commonwealth University

Chapters

  • 00:05Title
  • 02:04Longitudinal Muscle/Myenteric Plexus Harvesting
  • 04:42LMMP Digestion
  • 07:18Results: An in vitro Preparation of Isolated Enteric Neurons and Glia from the Myenteric Plexus of the Adult Mouse
  • 09:16Conclusion

Summary

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We demonstrate a cell culture protocol for the direct study of neuronal and glial components of the enteric nervous system. A neuron/glia mixed culture on coverslips is prepared from the myenteric plexus of adult mouse providing the ability to examine individual neuron and glia function by electrophysiology, immunohistochemical, etc.

Tags

Keywords: Enteric Nervous SystemMyenteric PlexusIn Vitro PreparationIsolated NeuronsEnteric GliaMouse IleumColonEnzymatic DigestionNeurite OutgrowthAction PotentialsGFAP StainingImmunohistochemistryElectrophysiologyCalcium ImagingSingle-cell PCRGenetically Modified Animals

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