需要订阅 JoVE 才能查看此. 登录或开始免费试用。
Isolation of Sensory Neurons of Aplysia californica for Patch Clamp Recordings of Glutamatergic Currents
12,136 Views
•
09:40 min
•
July 10, 2013
DOI:
Summary
Automatically generated
We describe the dissection of the nervous system of the marine sea hare Aplysia after anesthesia, the isolation of neurons for short term-tissue culture, and recordings of single cell ion currents via the patch clamp technique.
Read Article
Cite this Article
Fieber, L. A., Carlson, S. L., Kempsell, A. T., Greer, J. B., Schmale, M. C. Isolation of Sensory Neurons of Aplysia californica for Patch Clamp Recordings of Glutamatergic Currents. J. Vis. Exp. (77), e50543, doi:10.3791/50543 (2013).
Copy