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Isolation of Sensory Neurons of Aplysia californica for Patch Clamp Recordings of Glutamatergic Currents
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Isolation of Sensory Neurons of Aplysia californica for Patch Clamp Recordings of Glutamatergic Currents
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July 10, 2013
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We describe the dissection of the nervous system of the marine sea hare Aplysia after anesthesia, the isolation of neurons for short term-tissue culture, and recordings of single cell ion currents via the patch clamp technique.
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Fieber, L. A., Carlson, S. L., Kempsell, A. T., Greer, J. B., Schmale, M. C. Isolation of Sensory Neurons of Aplysia californica for Patch Clamp Recordings of Glutamatergic Currents. J. Vis. Exp. (77), e50543, doi:10.3791/50543 (2013).
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