Visualization and Analysis of mRNA Molecules Using Fluorescence In Situ Hybridization in Saccharomyces cerevisiae

R. Scott McIsaac; Sanford J. Silverman; Lance Parsons; Ping Xu; Ryan Briehof; Megan N. McClean; David Botstein

doi:10.3791/50382

/

Visualisierung und Analyse von mRNA Moleküle mittels Fluoreszenz In Situ Hybridisierung in Saccharomyces cerevisiae</em

JoVE Journal
Biology

This content is Free Access.

JoVE Journal Biology

Visualization and Analysis of mRNA Molecules Using Fluorescence In Situ Hybridization in Saccharomyces cerevisiae

Please note that all translations are automatically generated. Click here for the English version.

Visualisierung und Analyse von mRNA Moleküle mittels Fluoreszenz In Situ Hybridisierung in Saccharomyces cerevisiae</em

34,333 Views

•

07:00 min

•

June 14, 2013

DOI:

10.3791/50382-v

DOI:

10.3791/50382-v

•

07:00 min

June 14, 2013

•

3 Views

R. Scott McIsaac^1,2, Sanford J. Silverman¹, Lance Parsons¹, Ping Xu¹, Ryan Briehof¹, Megan N. McClean¹, David Botstein^1,3

¹The Lewis-Sigler Institute for Integrative Genomics,Princeton University, ²Graduate Program in Quantitative and Computational Biology,Princeton University, ³Department of Molecular Biology,Princeton University

Summary

Dieses Protokoll beschreibt ein experimentelles Verfahren für die Durchführung Fluorescence In situ Hybridisierung (FISH) zum Zählen mRNAs in einzelnen Zellen auf Einzel-Molekül-Auflösung.

Explore More Videos

Fluorescence In Situ Hybridization FISH MRNA Saccharomyces Cerevisiae Single-cell Analysis Single-molecule Spot Detection Gene Expression Visualization Quantification

Read Article

Visualisierung und Analyse von mRNA Moleküle mittels Fluoreszenz<em> In Situ</em> Hybridisierung in<em> Saccharomyces cerevisiae</em

Automatically Generated

Summary

Automatically generated

Explore More Videos