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Visualization and Analysis of mRNA Molecules Using Fluorescence In Situ Hybridization in Saccharomyces cerevisiae
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Visualization and Analysis of mRNA Molecules Using Fluorescence In Situ Hybridization in Saccharomyces cerevisiae

Visualization and Analysis of mRNA Molecules Using Fluorescence In Situ Hybridization in Saccharomyces cerevisiae

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07:00 min

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June 14, 2013

DOI:

10.3791/50382-v

DOI:
10.3791/50382-v

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07:00 min
June 14, 2013

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1The Lewis-Sigler Institute for Integrative Genomics,Princeton University, 2Graduate Program in Quantitative and Computational Biology,Princeton University, 3Department of Molecular Biology,Princeton University

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This protocol describes an experimental procedure for performing Fluorescence in situ Hybridization (FISH) for counting mRNAs in single cells at single-molecule resolution.

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Fluorescence In Situ HybridizationFISHMRNASaccharomyces CerevisiaeSingle-cell AnalysisSingle-moleculeSpot DetectionGene ExpressionVisualizationQuantification

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McIsaac, R. S., Silverman, S. J., Parsons, L., Xu, P., Briehof, R., McClean, M. N., Botstein, D. Visualization and Analysis of mRNA Molecules Using Fluorescence In Situ Hybridization in Saccharomyces cerevisiae. J. Vis. Exp. (76), e50382, doi:10.3791/50382 (2013).

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