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Visualization and Analysis of mRNA Molecules Using Fluorescence In Situ Hybridization in Saccharomyces cerevisiae
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Visualization and Analysis of mRNA Molecules Using Fluorescence In Situ Hybridization in Saccharomyces cerevisiae

Visualization and Analysis of mRNA Molecules Using Fluorescence In Situ Hybridization in Saccharomyces cerevisiae

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07:00 min

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June 14, 2013

DOI:

10.3791/50382-v

DOI:
10.3791/50382-v

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07:00 min
June 14, 2013

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, , , , , ,
1The Lewis-Sigler Institute for Integrative Genomics,Princeton University, 2Graduate Program in Quantitative and Computational Biology,Princeton University, 3Department of Molecular Biology,Princeton University

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This protocol describes an experimental procedure for performing Fluorescence in situ Hybridization (FISH) for counting mRNAs in single cells at single-molecule resolution.

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Fluorescence In Situ HybridizationFISHMRNASaccharomyces CerevisiaeSingle-cell AnalysisSingle-moleculeSpot DetectionGene ExpressionVisualizationQuantification

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Cite this Article

McIsaac, R. S., Silverman, S. J., Parsons, L., Xu, P., Briehof, R., McClean, M. N., Botstein, D. Visualization and Analysis of mRNA Molecules Using Fluorescence In Situ Hybridization in Saccharomyces cerevisiae. J. Vis. Exp. (76), e50382, doi:10.3791/50382 (2013).

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