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Undersøgelse af Makrofag Polarization Brug knoglemarvsafledte makrofager
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Immunology and Infection
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JoVE Journal Immunology and Infection
Investigation of Macrophage Polarization Using Bone Marrow Derived Macrophages

Undersøgelse af Makrofag Polarization Brug knoglemarvsafledte makrofager

DOI:
10.3791/50323-v

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10:07 min

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June 23, 2013

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, , , ,
1Department of Animal Science,Texas A&M University, 2Department of Biology,Texas A&M University, 3Department of Physiology and Pharmacology, College of Veterinary Medicine and Biomedical Sciences,Texas A&M University

Chapters

  • 00:05Title
  • 01:36Isolation of Bone Marrow Cells
  • 03:23Induction BMDM Formation
  • 04:17BMDM Polarized Activation
  • 06:11Representative Analysis of BMDM
  • 09:19Conclusion

Summary

Automatic Translation

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I artiklen beskrives en let let adaptiv<em> In vitro</em> Model til at undersøge makrofag polarisering. I nærvær af GM-CSF/M-CSF er hæmatopoietiske stamceller / stamceller fra knoglemarven ledes ind monocytær differentiering, efterfulgt af M1 eller M2 stimulation. Aktiveringen status kan spores af ændringer i celleoverfladeantigener, genekspression og celle signalveje.

Tags

Macrophage PolarizationBone Marrow Derived MacrophagesGM-CSFM-CSFMonocytic DifferentiationM1 PolarizationM2 PolarizationCell Surface AntigensGene ExpressionCell Signaling Pathways

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