/

Immunology and Infection

/

使用骨髓源性巨噬细胞巨噬细胞极化的调查

JoVE Journal
Immunology and Infection

A subscription to JoVE is required to view this content. Sign in or start your free trial.

JoVE Journal Immunology and Infection

Investigation of Macrophage Polarization Using Bone Marrow Derived Macrophages

Please note that all translations are automatically generated. Click here for the English version.

使用骨髓源性巨噬细胞巨噬细胞极化的调查

DOI:

10.3791/50323-v

•

10:07 min

•

June 23, 2013

•

Wei Ying, Patali S. Cheruku, Fuller W. Bazer, Stephen H. Safe, Beiyan Zhou

¹Department of Animal Science,Texas A&M University, ²Department of Biology,Texas A&M University, ³Department of Physiology and Pharmacology, College of Veterinary Medicine and Biomedical Sciences,Texas A&M University

Chapters

00:05Title
01:36Isolation of Bone Marrow Cells
03:23Induction BMDM Formation
04:17BMDM Polarized Activation
06:11Representative Analysis of BMDM
09:19Conclusion

Summary

Automatic Translation

本文介绍了一种自适应容易容易<em>在体外</em>模型，探讨巨噬细胞极化。在存在下GM-CSF/M-CSF，从骨髓中的造血干/祖细胞定向分化成单核细胞，由M1或M2的刺激。激活状态可以跟踪的细胞表面抗原，基因表达和细胞信号转导通路的变化。

Tags

Macrophage Polarization Bone Marrow Derived Macrophages GM-CSF M-CSF Monocytic Differentiation M1 Polarization M2 Polarization Cell Surface Antigens Gene Expression Cell Signaling Pathways

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

弓形虫囊肿壁形成激活骨髓衍生的巨噬细胞和Bradyzoite条件

小鼠骨髓来源的树突状细胞与跟踪研究的Qdot纳米晶的生成及标签

一个<em在体外</em>模型研究人类巨噬细胞分化和极化的异质性

骨髓衍生的巨噬细胞产

隔离和小鼠骨髓的静脉注射单核细胞衍生

偏光M1和M2骨髓来源的巨噬细胞的使用实时外流量分析代谢表征

鼠后肢长骨解剖和骨髓隔离

代和GM-CSF的鉴定源性肺泡状巨噬细胞和树突状细胞从小鼠骨髓

巨噬细胞集落刺激因子分化的人单核细胞衍生的巨噬细胞的培养

抗体药物对小鼠骨髓及腹腔巨噬细胞活化作用的评价

Read Article