Simultaneous Pre- and Post-synaptic Electrophysiological Recording from Xenopus Nerve-muscle Co-cultures

Bruce Yazejian; Rita M. Yazejian; Rachel Einarsson; Alan D. Grinnell

doi:10.3791/50253

Journal

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Neuroscience

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Simultanea di pre-e post-sinaptica registrazione elettrofisiologica da Xenopus Nervo-muscolo Co-colture

JoVE Journal
Neuroscience

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JoVE Journal Neuroscience

Simultaneous Pre- and Post-synaptic Electrophysiological Recording from Xenopus Nerve-muscle Co-cultures

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Simultanea di pre-e post-sinaptica registrazione elettrofisiologica da Xenopus Nervo-muscolo Co-colture

10,731 Views

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08:13 min

•

March 11, 2013

DOI:

10.3791/50253-v

DOI:

10.3791/50253-v

•

08:13 min

March 11, 2013

•

1 Views

Bruce Yazejian¹, Rita M. Yazejian¹, Rachel Einarsson², Alan D. Grinnell¹

¹Department of Physiology,David Geffen School of Medicine at UCLA, ²Natural Science Division,Pepperdine University

Summary

Questo video mostra le procedure utilizzate per coltivare colture primarie di embrionale Xenopus Cellule nervose e muscolari e l'utilità di questo preparato per la realizzazione simultanea di pre-e post-sinaptica registrazioni patch clamp.

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Simultanea di pre-e post-sinaptica registrazione elettrofisiologica da<em> Xenopus</em> Nervo-muscolo Co-colture

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