This video demonstrates the procedures used to grow primary cultures of embryonic Xenopus nerve and muscle cells and the usefulness of this preparation for making simultaneous pre- and post-synaptic patch clamp recordings.
Yazejian, B., Yazejian, R. M., Einarsson, R., Grinnell, A. D. Simultaneous Pre- and Post-synaptic Electrophysiological Recording from Xenopus Nerve-muscle Co-cultures. J. Vis. Exp. (73), e50253, doi:10.3791/50253 (2013).