Simultaneous Pre- and Post-synaptic Electrophysiological Recording from Xenopus Nerve-muscle Co-cultures

Bruce Yazejian; Rita M. Yazejian; Rachel Einarsson; Alan D. Grinnell

doi:10.3791/50253

Journal

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Neuroscience

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Simultanée pré-et post-synaptique enregistrement électrophysiologique de Xenopus Nerf-muscle co-cultures

JoVE Journal
Neuroscience

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JoVE Journal Neuroscience

Simultaneous Pre- and Post-synaptic Electrophysiological Recording from Xenopus Nerve-muscle Co-cultures

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Simultanée pré-et post-synaptique enregistrement électrophysiologique de Xenopus Nerf-muscle co-cultures

10,731 Views

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08:13 min

•

March 11, 2013

DOI:

10.3791/50253-v

DOI:

10.3791/50253-v

•

08:13 min

March 11, 2013

•

1 Views

Bruce Yazejian¹, Rita M. Yazejian¹, Rachel Einarsson², Alan D. Grinnell¹

¹Department of Physiology,David Geffen School of Medicine at UCLA, ²Natural Science Division,Pepperdine University

Summary

Cette vidéo montre les procédures utilisées pour cultiver des cultures primaires d'embryon de Xenopus</emNerf> et les cellules musculaires et l'utilité de cette préparation pour faire simultanées pré-et post-synaptique des enregistrements de patch-clamp.

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Simultanée pré-et post-synaptique enregistrement électrophysiologique de<em> Xenopus</em> Nerf-muscle co-cultures

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Summary

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