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Mitokondri ilişkili ER Membranlar (MAM) ve Glycosphingolipid zenginleştirilmiştir Microdomains (GEM'lerle): Fare Beyin izolasyon
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Neuroscience
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JoVE Journal Neuroscience
Mitochondria-associated ER Membranes (MAMs) and Glycosphingolipid Enriched Microdomains (GEMs): Isolation from Mouse Brain
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Mitokondri ilişkili ER Membranlar (MAM) ve Glycosphingolipid zenginleştirilmiştir Microdomains (GEM'lerle): Fare Beyin izolasyon

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24,093 Views

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10:32 min

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March 04, 2013

DOI:

10.3791/50215-v

DOI:
10.3791/50215-v

•

10:32 min
March 04, 2013

•

10 Views
, ,
1Department of Genetics,St Jude Children’s Research Hospital

Summary

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Bu işlem yetişkin fare beyin mitokondri ilişkili ER membranlar veya MAM ve MAM ve mitokondriyal hazırlıkları glycosphingolipid zenginleştirilmiş microdomain fraksiyonları izole etmek için nasıl göstermektedir.

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Annunziata, I., Patterson, A., d'Azzo, A. Mitochondria-associated ER Membranes (MAMs) and Glycosphingolipid Enriched Microdomains (GEMs): Isolation from Mouse Brain. J. Vis. Exp. (73), e50215, doi:10.3791/50215 (2013).

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