This procedure illustrates how to isolate from the adult mouse brain the mitochondria-associated ER membranes or MAMs and the glycosphingolipid-enriched microdomain fractions from MAMs and mitochondrial preparations.
Annunziata, I., Patterson, A., d'Azzo, A. Mitochondria-associated ER Membranes (MAMs) and Glycosphingolipid Enriched Microdomains (GEMs): Isolation from Mouse Brain. J. Vis. Exp. (73), e50215, doi:10.3791/50215 (2013).