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Mitochondria-associated ER Membranes (MAMs) and Glycosphingolipid Enriched Microdomains (GEMs): Isolation from Mouse Brain
JoVE Journal
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JoVE Journal Nörobilim
Mitochondria-associated ER Membranes (MAMs) and Glycosphingolipid Enriched Microdomains (GEMs): Isolation from Mouse Brain

Mitochondria-associated ER Membranes (MAMs) and Glycosphingolipid Enriched Microdomains (GEMs): Isolation from Mouse Brain

24,062 Views

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10:32 min

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March 04, 2013

DOI:

10.3791/50215-v

DOI:
10.3791/50215-v

•

10:32 min
March 04, 2013

•

10 Views
, ,
1Genetik Bölümü,St Jude Children’s Research Hospital

Özet

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This procedure illustrates how to isolate from the adult mouse brain the mitochondria-associated ER membranes or MAMs and the glycosphingolipid-enriched microdomain fractions from MAMs and mitochondrial preparations.

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Annunziata, I., Patterson, A., d'Azzo, A. Mitochondria-associated ER Membranes (MAMs) and Glycosphingolipid Enriched Microdomains (GEMs): Isolation from Mouse Brain. J. Vis. Exp. (73), e50215, doi:10.3791/50215 (2013).

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