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Klinische toepassing van<em> Sleeping Beauty</em> En Kunstmatige antigeen presenterende cellen genetisch te wijzigen T-cellen uit perifeer en navelstrengbloed
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Clinical Application of Sleeping Beauty and Artificial Antigen Presenting Cells to Genetically Modify T Cells from Peripheral and Umbilical Cord Blood
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Klinische toepassing van<em> Sleeping Beauty</em> En Kunstmatige antigeen presenterende cellen genetisch te wijzigen T-cellen uit perifeer en navelstrengbloed

Nederlands

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18,099 Views

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09:29 min

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February 01, 2013

DOI:

10.3791/50070-v

DOI:
10.3791/50070-v

•

09:29 min
February 01, 2013

•

3 Views
, , , , , , , ,
1Division of Pediatrics,U.T. MD Anderson Cancer Center, 2Department of Stem Cell Transplantation and Cellular Therapy,U.T. MD Anderson Cancer Center

Summary

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T cellen die een CD19-antigen specifieke chimere receptor (CAR) worden toegediend als onderzoek behandeling van B-cel maligniteiten in de eerste-in-humane gentherapie trials. We beschrijven genetische modificatie van T cellen met behulp van de<em> Sleeping Beauty</em> (SB) systeem CD19-specifieke CAR en selectieve voortplanting te introduceren op designer CD19 + kunstmatige antigeen presenterende cellen.

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Sleeping BeautyArtificial Antigen Presenting CellsGenetic ModificationT CellsPeripheral BloodUmbilical Cord BloodCAR T CellsCD19Gene TherapyImmunotherapyTumor-associated AntigensClinical Application

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Cite this Article

Huls, M. H., Figliola, M. J., Dawson, M. J., Olivares, S., Kebriaei, P., Shpall, E. J., Champlin, R. E., Singh, H., Cooper, L. J. Clinical Application of Sleeping Beauty and Artificial Antigen Presenting Cells to Genetically Modify T Cells from Peripheral and Umbilical Cord Blood. J. Vis. Exp. (72), e50070, doi:10.3791/50070 (2013).

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