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Clinical Application of Sleeping Beauty and Artificial Antigen Presenting Cells to Genetically Modify T Cells from Peripheral and Umbilical Cord Blood
JoVE Journal
免疫学と感染
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JoVE Journal 免疫学と感染
Clinical Application of Sleeping Beauty and Artificial Antigen Presenting Cells to Genetically Modify T Cells from Peripheral and Umbilical Cord Blood

Clinical Application of Sleeping Beauty and Artificial Antigen Presenting Cells to Genetically Modify T Cells from Peripheral and Umbilical Cord Blood

18,099 Views

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09:29 min

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February 01, 2013

DOI:

10.3791/50070-v

DOI:
10.3791/50070-v

•

09:29 min
February 01, 2013

•

3 Views
, , , , , , , ,
1Division of Pediatrics,U.T. MD Anderson Cancer Center, 2Department of Stem Cell Transplantation and Cellular Therapy,U.T. MD Anderson Cancer Center

概要

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T cells expressing a CD19-specific chimeric antigen receptor (CAR) are infused as investigational treatment of B-cell malignancies in our first-in-human gene therapy trials. We describe genetic modification of T cells using the Sleeping Beauty (SB) system to introduce CD19-specific CAR and selective propagation on designer CD19+ artificial antigen presenting cells.

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Sleeping BeautyArtificial Antigen Presenting CellsGenetic ModificationT CellsPeripheral BloodUmbilical Cord BloodCAR T CellsCD19Gene TherapyImmunotherapyTumor-associated AntigensClinical Application

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Cite this Article

Huls, M. H., Figliola, M. J., Dawson, M. J., Olivares, S., Kebriaei, P., Shpall, E. J., Champlin, R. E., Singh, H., Cooper, L. J. Clinical Application of Sleeping Beauty and Artificial Antigen Presenting Cells to Genetically Modify T Cells from Peripheral and Umbilical Cord Blood. J. Vis. Exp. (72), e50070, doi:10.3791/50070 (2013).

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