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Neuroscience
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神经元对神经胶质细胞在微流控文化互动平台(MCP)的神经元轴突和神经胶质细胞共培养系统的影像学分析
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Neuroscience
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JoVE Journal
Neuroscience
Imaging Analysis of Neuron to Glia Interaction in Microfluidic Culture Platform (MCP)-based Neuronal Axon and Glia Co-culture System
Please note that all translations are automatically generated.
Click here for the English version.
神经元对神经胶质细胞在微流控文化互动平台(MCP)的神经元轴突和神经胶质细胞共培养系统的影像学分析
DOI:
10.3791/4448-v
•
09:34 min
•
October 14, 2012
•
Haruki Higashimori
,
Yongjie Yang
2
1
Department of Neuroscience
,
Tufts University
,
2
Neuroscience Program
,
Tufts Sackler School of Graduate Biomedical Sciences
Chapters
00:05
Title
01:27
Assembly of the Microfluidic Culture Chamber (MCP)
02:43
Preparation of Neuronal Culture
03:33
Induction of Neuronal Axons in Assembled MCP
04:48
A Compartmentalized Co-culture System: Adding Cultured Astrocytes
06:15
Results: Axon-induced GLT1 Promoter Activation in Astrocytes
09:07
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
本研究介绍了一种新的神经元轴突和神经胶质细胞共培养(ASTRO)平台的程序。在此共培养体系中的一个单一的轴突(及单一的神经胶质细胞)之间的直接相互作用,操纵变得可行,允许机械分析的相互神经胶质信令。
Tags
Imaging Analysis
Neuron
Glia Interaction
Microfluidic Culture Platform
MCP-based Neuronal Axon And Glia Co-culture System
Signaling Pathways
Central Nervous System (CNS)
Neuron And Glia Mixed Cultures
Neuronal Compartments
Axon
Dendrite
Soma
Culture System
Soluble Factors
Direct Membrane Contact Signals
Single Axon
Glial Cell
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