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A Cell Culture Model for Studying the Role of Neuron-Glia Interactions in Ischemia
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Neuroscience
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JoVE Journal Neuroscience
A Cell Culture Model for Studying the Role of Neuron-Glia Interactions in Ischemia

A Cell Culture Model for Studying the Role of Neuron-Glia Interactions in Ischemia

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9,037 Views

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11:36 min

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November 14, 2020

DOI:

10.3791/61388-v

DOI:
10.3791/61388-v

•

11:36 min
November 14, 2020

•

12 Views
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1Centro de Investigação em Ciências da Saúde (CICS-UBI),Universidade da Beira Interior, 2Faculdade de Ciências da Saúde,Universidade da Beira Interior

Summary

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Here, we present a simple approach using specific culture media that allows the establishment of neuron- and astrocyte-enriched cultures, or neuron-glia cultures from the embryonic cortex, with high yield and reproducibility.

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Cell CultureNeuron-glia InteractionsIschemiaAstrocytesNeuronsOxygen And Glucose DeprivationRat Embryonic Cortex

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Cite this Article

Gava-Junior, G., Roque, C., Mendes-Oliveira, J., Bernardino, A. C., Serrenho, I., Pires, J. P., Baltazar, G. A Cell Culture Model for Studying the Role of Neuron-Glia Interactions in Ischemia. J. Vis. Exp. (165), e61388, doi:10.3791/61388 (2020).

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