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Rearing and Injection of Manduca sexta Larvae to Assess Bacterial Virulence
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Rearing and Injection of Manduca sexta Larvae to Assess Bacterial Virulence

Rearing and Injection of Manduca sexta Larvae to Assess Bacterial Virulence

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09:04 min

December 11, 2012

DOI:

09:04 min
December 11, 2012

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Summary

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The method described here utilizes direct injection of entomopathogenic bacteria into the hemocoel of Manduca sexta insect larvae. M. sexta is a commercially available and well-studied insect. Thus, this method represents a simple approach to analyzing host-bacterial interactions from the perspective of one or both partners.

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