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Rearing and Injection of Manduca sexta Larvae to Assess Bacterial Virulence
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Rearing and Injection of Manduca sexta Larvae to Assess Bacterial Virulence
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December 11, 2012
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The method described here utilizes direct injection of entomopathogenic bacteria into the hemocoel of Manduca sexta insect larvae. M. sexta is a commercially available and well-studied insect. Thus, this method represents a simple approach to analyzing host-bacterial interactions from the perspective of one or both partners.
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Manduca SextaTobacco HornwormAgricultural PestSolanaceous PlantsEntomopathogenic BacteriaInsect Immune SystemGenome SequenceModel OrganismHost-microbe InteractionsPathogenesisLarvae InjectionVirulence CharacteristicsBacterial SpeciesMutantsTime To Insect DeathXenorhabdus SpeciesPhotorhabdus SpeciesNematode VectorsInsect Hemolymph
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Hussa, E., Goodrich-Blair, H. Rearing and Injection of Manduca sexta Larvae to Assess Bacterial Virulence. J. Vis. Exp. (70), e4295, doi:10.3791/4295 (2012).
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