The method described here utilizes direct injection of entomopathogenic bacteria into the hemocoel of Manduca sexta insect larvae. M. sexta is a commercially available and well-studied insect. Thus, this method represents a simple approach to analyzing host-bacterial interactions from the perspective of one or both partners.
Hussa, E., Goodrich-Blair, H. Rearing and Injection of Manduca sexta Larvae to Assess Bacterial Virulence. J. Vis. Exp. (70), e4295, doi:10.3791/4295 (2012).