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Immunology and Infection

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プライマリーヒトT細胞のpiggyBacトランスポゾンシステムの変更

JoVE Journal
Immunology and Infection

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JoVE Journal Immunology and Infection

piggyBac Transposon System Modification of Primary Human T Cells

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プライマリーヒトT細胞のpiggyBacトランスポゾンシステムの変更

16,947 Views

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10:02 min

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November 05, 2012

DOI:

DOI:

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10:02 min

November 05, 2012

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7 Views

Sunandan Saha^1,2, Yozo Nakazawa³, Leslie E. Huye^4,5, Joseph E. Doherty^2,6, Daniel L. Galvan², Cliona M. Rooney^4,5,7, Matthew H. Wilson^1,2,4,8

¹Program in Translational Biology and Molecular Medicine,Baylor College of Medicine , ²Department of Medicine, Division of Nephrology,Baylor College of Medicine , ³Department of Immunology and Pathology,Shinshu University School of Medicine, ⁴Center for Cell and Gene Therapy,Baylor College of Medicine , ⁵Department of Pediatrics,Baylor College of Medicine , ⁶Program in Cell and Molecular Biology,Baylor College of Medicine , ⁷Department of Molecular Virology and Microbiology,Baylor College of Medicine , ⁸Michael E. DeBakey VA Medical Center

Summary

我々は、遺伝的に非ウイルスを用いて導入遺伝子を持つプライマリヒトT細胞を変更する方法を説明します<em> piggyBac</em>トランスポゾンシステム。 T細胞は、使用して変更された<em> piggyBac</em>トランスポゾンシステムは安定した導入遺伝子の発現を示す。

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