このコンテンツを視聴するには、JoVE 購読が必要です。 サインイン又は無料トライアルを申し込む。
piggyBac Transposon System Modification of Primary Human T Cells
16,949 Views
•
10:02 min
•
November 05, 2012
DOI:
1Program in Translational Biology and Molecular Medicine,Baylor College of Medicine , 2Department of Medicine, Division of Nephrology,Baylor College of Medicine , 3Department of Immunology and Pathology,Shinshu University School of Medicine, 4Center for Cell and Gene Therapy,Baylor College of Medicine , 5小児科,Baylor College of Medicine , 6Program in Cell and Molecular Biology,Baylor College of Medicine , 7Department of Molecular Virology and Microbiology,Baylor College of Medicine , 8Michael E. DeBakey VA Medical Center
概要
Automatically generated
We describe a method to genetically modify primary human T cells with a transgene using the non-viral piggyBac transposon system. T cells modified to using the piggyBac transposon system exhibit stable transgene expression.
Read Article
Cite this Article
Saha, S., Nakazawa, Y., Huye, L. E., Doherty, J. E., Galvan, D. L., Rooney, C. M., Wilson, M. H. piggyBac Transposon System Modification of Primary Human T Cells. J. Vis. Exp. (69), e4235, doi:10.3791/4235 (2012).
Copy