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piggyBac Transposon System Modification of Primary Human T Cells
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10:02 min
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November 05, 2012
DOI:
1Program in Translational Biology and Molecular Medicine,Baylor College of Medicine , 2Department of Medicine, Division of Nephrology,Baylor College of Medicine , 3Department of Immunology and Pathology,Shinshu University School of Medicine, 4Center for Cell and Gene Therapy,Baylor College of Medicine , 5Department of Pediatrics,Baylor College of Medicine , 6Program in Cell and Molecular Biology,Baylor College of Medicine , 7Department of Molecular Virology and Microbiology,Baylor College of Medicine , 8Michael E. DeBakey VA Medical Center
Summary
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We describe a method to genetically modify primary human T cells with a transgene using the non-viral piggyBac transposon system. T cells modified to using the piggyBac transposon system exhibit stable transgene expression.
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Saha, S., Nakazawa, Y., Huye, L. E., Doherty, J. E., Galvan, D. L., Rooney, C. M., Wilson, M. H. piggyBac Transposon System Modification of Primary Human T Cells. J. Vis. Exp. (69), e4235, doi:10.3791/4235 (2012).
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