A Modified EPA Method 1623 that Uses Tangential Flow Hollow-fiber Ultrafiltration and Heat Dissociation Steps to Detect Waterborne Cryptosporidium and Giardia spp.

Eric R. Rhodes; Leah Fohl Villegas; Nancy J. Shaw; Carrie Miller; Eric N. Villegas

doi:10.3791/4177

Journal

/

Immunology and Infection

/

一种改进的EPA方法1623使用切向流中空纤维超滤和热解离步骤检测水性隐和贾第虫属。</em

JoVE Journal
Immunology and Infection

This content is Free Access.

JoVE Journal Immunology and Infection

A Modified EPA Method 1623 that Uses Tangential Flow Hollow-fiber Ultrafiltration and Heat Dissociation Steps to Detect Waterborne Cryptosporidium and Giardia spp.

Please note that all translations are automatically generated. Click here for the English version.

一种改进的EPA方法1623使用切向流中空纤维超滤和热解离步骤检测水性隐和贾第虫属。</em

DOI:

10.3791/4177-v

•

12:11 min

•

July 09, 2012

•

Eric R. Rhodes, Leah Fohl Villegas, Nancy J. Shaw, Carrie Miller, Eric N. Villegas

¹National Exposure Research Laboratory,Office of Research and Development, US Environmental Protection Agency, ²Shaw Environmental & Infrastructure, ³Office of Ground Water and Drinking Water,US Environmental Protection Agency

Chapters

00:05Title
01:18Tangential Flow Hollow-fiber Ultrafiltration Procedure
05:08Immunomagnetic Separation Procedure
08:58Staining and Examination
10:20Results: Visualization of Giardia cysts and Cryptosporidium oocysts
11:40Conclusion

Summary

Automatic Translation

本协议描述了作为替代水性的检测步骤使用的中空纤维切向流超滤样品浓缩系统和热分解隐和贾第物种通过采用EPA Method 1623。

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