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定向分化诱导性多潜能干细胞向T淋巴细胞
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Immunology and Infection
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JoVE Journal Immunology and Infection
Directed Differentiation of Induced Pluripotent Stem Cells towards T Lymphocytes
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定向分化诱导性多潜能干细胞向T淋巴细胞

DOI:
10.3791/3986-v

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12:47 min

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May 14, 2012

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, , ,
1Department of Microbiology and Immunology,Pennsylvania State University College of Medicine

Chapters

  • 00:05Title
  • 01:10Cell Culture and In vitro Programming
  • 02:15In vivo Maturation of Partially Differentiated iPS Cells
  • 03:36In vivo Programming
  • 04:51Adoptive Transfer and Peptide Stimulation
  • 05:50In vivo Killing Assay
  • 06:47Intraperitoneal Tumor Cell Count and Infiltrating Tumor Cell Identification
  • 08:43Results: In vivo Differentiation of TCR Gene-transduced iPS Cells into Functional Ag-specific CTLs
  • 12:25Conclusion

Summary

Automatic Translation

Automatic Translation

诱导多能干细胞（iPS）细胞，T淋巴细胞的生成，给出了一个使用基于T细胞免疫治疗的胚胎干细胞的替代方法。该方法表明，利用任<em>在体外</em>或<em>在体内</em>感应系统，iPS细胞能够分化成传统和抗原特异性T淋巴细胞。

Tags

Directed DifferentiationInduced Pluripotent Stem CellsT LymphocytesAdoptive Cell TransferAntigen-specific CD8+ Cytotoxic T LymphocytesT Cell ReceptorsCytotoxinsCytokinesHighly Reactive CTLsImmunotherapyHomeostatic CytokinesACT-based TherapiesTCR TransductionSelf-renewable Stem CellsImmune ReconstitutionEmbryonic Stem CellsHematopoietic Stem CellsIn Vitro Cell CultureIPS Cell TechnologyGene Delivery

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