È necessario avere un abbonamento a JoVE per visualizzare questo. Accedi o inizia la tua prova gratuita.
Single-cell Profiling of Developing and Mature Retinal Neurons
14,001 Views
•
10:20 min
•
April 19, 2012
DOI:
Summary
Automatically generated
A method for the isolation of single retinal cells and subsequent amplification of their cDNAs is described. Single-cell transcriptomics reveals the degree of cellular heterogeneity present in a tissue and uncovers new marker genes for rare cell populations. The accompanying protocol can be adjusted to suit many different cell types.
Explore More Videos
Single-cell ProfilingDeveloping Retinal NeuronsMature Retinal NeuronsCell-type Specific MarkersGene Expression ProgramsIndividual CellsTransient Gene Expression ProgramsCellular DiversityCentral Nervous SystemDistinct Cell TypesComplex Neural CircuitsRare Neuronal TypesMolecular ClassificationAnalysis Of Cell TypesNervous SystemMechanisms Of Neural Development
Read Article
Cite this Article
Goetz, J. J., Trimarchi, J. M. Single-cell Profiling of Developing and Mature Retinal Neurons. J. Vis. Exp. (62), e3824, doi:10.3791/3824 (2012).
Copy