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Single-cell Profiling of Developing and Mature Retinal Neurons
Journal JoVE
Neurosciences
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Journal JoVE Neurosciences
Single-cell Profiling of Developing and Mature Retinal Neurons

Single-cell Profiling of Developing and Mature Retinal Neurons

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10:20 min

April 19, 2012

DOI:

10:20 min
April 19, 2012

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Summary

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A method for the isolation of single retinal cells and subsequent amplification of their cDNAs is described. Single-cell transcriptomics reveals the degree of cellular heterogeneity present in a tissue and uncovers new marker genes for rare cell populations. The accompanying protocol can be adjusted to suit many different cell types.

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