/
/
Uno screening genetico per isolare Toxoplasma gondii Host-cell Egress Mutants
A subscription to JoVE is required to view this content. Sign in or start your free trial.
12,898 Views
•
14:56 min
•
February 08, 2012
DOI:
Summary
Automatically generated
Forward genetica è un metodo efficace per svelare il livello molecolare di come<em> Toxoplasma</em> Egresses dalla sua cellula ospite. I protocolli sono fornite mutagenizzare chimicamente parassiti, per arricchire mutanti con difetti in uscita indotta, e convalidare il fenotipo di mutanti clonati.
Explore More Videos
Genetic ScreenToxoplasma GondiiHost-cell Egress MutantsObligate Intracellular ParasiteLytic Replication CycleActive InvasionReplication Inside Host CellActive EgressMolecular LevelSignaling PathwaysPharmacological AgentsTriggers Of EgressIntracellular Ca2+Candidate Gene ApproachPlant Like Calcium Dependent Protein Kinase (CDPK)(chemical) Genetic ApproachesScreen For Mutant ParasitesDefect In Host Cell EgressChemical MutagenesisN-ethyl-N-nitrosourea (ENU)
Read Article
Cite this Article
Coleman, B. I., Gubbels, M. A Genetic Screen to Isolate Toxoplasma gondii Host-cell Egress Mutants. J. Vis. Exp. (60), e3807, doi:10.3791/3807 (2012).
Copy