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A Genetic Screen to Isolate Toxoplasma gondii Host-cell Egress Mutants
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A Genetic Screen to Isolate Toxoplasma gondii Host-cell Egress Mutants
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February 08, 2012
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Forward genetics is a powerful method to unravel the molecular level of how Toxoplasma egresses from its host cell. Protocols are provided to chemically mutagenize parasites, enrich for mutants with defects in induced egress, and validate the phenotype of cloned mutants.
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Coleman, B. I., Gubbels, M. A Genetic Screen to Isolate Toxoplasma gondii Host-cell Egress Mutants. J. Vis. Exp. (60), e3807, doi:10.3791/3807 (2012).
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