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A Genetic Screen to Isolate Toxoplasma gondii Host-cell Egress Mutants
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February 08, 2012
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Forward genetics is a powerful method to unravel the molecular level of how Toxoplasma egresses from its host cell. Protocols are provided to chemically mutagenize parasites, enrich for mutants with defects in induced egress, and validate the phenotype of cloned mutants.
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Genetic ScreenToxoplasma GondiiHost-cell Egress MutantsObligate Intracellular ParasiteLytic Replication CycleActive InvasionReplication Inside Host CellActive EgressMolecular LevelSignaling PathwaysPharmacological AgentsTriggers Of EgressIntracellular Ca2+Candidate Gene ApproachPlant Like Calcium Dependent Protein Kinase (CDPK)(chemical) Genetic ApproachesScreen For Mutant ParasitesDefect In Host Cell EgressChemical MutagenesisN-ethyl-N-nitrosourea (ENU)
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Coleman, B. I., Gubbels, M. A Genetic Screen to Isolate Toxoplasma gondii Host-cell Egress Mutants. J. Vis. Exp. (60), e3807, doi:10.3791/3807 (2012).
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