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Profiling Thiolredox Proteome Mit Isotopen-Massenspektrometrie Tagging
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Profiling Thiol Redox Proteome Using Isotope Tagging Mass Spectrometry
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Profiling Thiolredox Proteome Mit Isotopen-Massenspektrometrie Tagging

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15,879 Views

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12:07 min

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March 24, 2012

DOI:

10.3791/3766-v

DOI:
10.3791/3766-v

•

12:07 min
March 24, 2012

•

2 Views
, , ,
1Plant Molecular and Cellular Biology Program,University of Florida , 2Department of Biology,University of Florida , 3Interdisciplinary Center for Biotechnology Research,University of Florida , 4Genetics Institute,University of Florida

Summary

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Reaktive Sauerstoffspezies Niveau angehoben wird, wenn Zellen begegnen Stressbedingungen. Hier zeigen wir das Beispiel von 3'-3 'Diaminobenzidin-Färbung sowie cysTMT Kennzeichnung und Massenspektrometrie, um die Redox-Proteom im Profil<em> Pseudomonas syringae</em> Behandelt Tomatenblättern.

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Parker, J., Zhu, N., Zhu, M., Chen, S. Profiling Thiol Redox Proteome Using Isotope Tagging Mass Spectrometry. J. Vis. Exp. (61), e3766, doi:10.3791/3766 (2012).

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