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Profiling Thiol Redox Proteome Using Isotope Tagging Mass Spectrometry
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生物学
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JoVE Journal 生物学
Profiling Thiol Redox Proteome Using Isotope Tagging Mass Spectrometry

Profiling Thiol Redox Proteome Using Isotope Tagging Mass Spectrometry

15,892 Views

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12:07 min

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March 24, 2012

DOI:

10.3791/3766-v

DOI:
10.3791/3766-v

•

12:07 min
March 24, 2012

•

2 Views
, , ,
1Plant Molecular and Cellular Biology Program,University of Florida , 2生物学科,University of Florida , 3Interdisciplinary Center for Biotechnology Research,University of Florida , 4Genetics Institute,University of Florida

概要

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Reactive oxygen species level is elevated when cells encounter stress conditions. Here we show the example of 3'-3' diaminobenzidine staining as well as cysTMT labeling and mass spectrometry to profile the redox proteome in Pseudomonas syringae treated tomato leaves.

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Thiol Redox ProteomeIsotope Tagging Mass SpectrometryPseudomonas Syringae Pv. TomatoBacterial Speck DiseaseSolanum LycopersicumBrassica SpeciesArabidopsis ThalianaReactive Oxygen Species (ROS)Hydrogen PeroxideDAB StainingRedox Status Of ProteinsCysteine OxidationTandem Mass Tag (TMT) ReagentsCysteine-reactive TMT (cysTMT) Tags

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Cite this Article

Parker, J., Zhu, N., Zhu, M., Chen, S. Profiling Thiol Redox Proteome Using Isotope Tagging Mass Spectrometry. J. Vis. Exp. (61), e3766, doi:10.3791/3766 (2012).

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