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Profiling Thiol Redox Proteome Using Isotope Tagging Mass Spectrometry
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JoVE Journal Biyoloji
Profiling Thiol Redox Proteome Using Isotope Tagging Mass Spectrometry

Profiling Thiol Redox Proteome Using Isotope Tagging Mass Spectrometry

DOI:
10.3791/3766-v

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12:07 min

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March 24, 2012

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, , ,
1Plant Molecular and Cellular Biology Program,Florida Üniversitesi , 2Biyoloji Bölümü,Florida Üniversitesi , 3Interdisciplinary Center for Biotechnology Research,Florida Üniversitesi , 4Genetics Institute,Florida Üniversitesi

Bölümler

  • 00:05Başlık
  • 02:07Inoculation of Tomato
  • 03:13Protein Extraction
  • 04:21Sample Preparation and Peptide Labeling with cysTMT
  • 06:07Removal of Non-reacted Tag Sample Fractionation
  • 07:44Sample Enrichment of cysTMT-labeled Peptides
  • 08:43Mass Spectrometry Analysis
  • 10:02CysTMT Results: Profiling Thiol Redox Proteome of Pseudomonas syringae in Tomato
  • 11:11Conclusion

Özet

Otomatik Çeviri

Otomatik Çeviri

Reactive oxygen species level is elevated when cells encounter stress conditions. Here we show the example of 3'-3' diaminobenzidine staining as well as cysTMT labeling and mass spectrometry to profile the redox proteome in Pseudomonas syringae treated tomato leaves.

Etiketler

Thiol Redox ProteomeIsotope Tagging Mass SpectrometryPseudomonas Syringae Pv. TomatoBacterial Speck DiseaseSolanum LycopersicumBrassica SpeciesArabidopsis ThalianaReactive Oxygen Species (ROS)Hydrogen PeroxideDAB StainingRedox Status Of ProteinsCysteine OxidationTandem Mass Tag (TMT) ReagentsCysteine-reactive TMT (cysTMT) Tags

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