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Generation of Organotypic Raft Cultures from Primary Human Keratinocytes
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February 22, 2012
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An in vitro method to mimic in vivo epithelial differentiation is described. Many viruses target epithelial cells as part of their viral life cycle, and this method provides a means of examining virus:host interactions that more closely resembles that which occurs in vivo. This technique can be used with primary keratinocytes, established cell lines, as well as normal or diseased biopsy tissue.
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Anacker, D., Moody, C. Generation of Organotypic Raft Cultures from Primary Human Keratinocytes. J. Vis. Exp. (60), e3668, doi:10.3791/3668 (2012).
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