このコンテンツを視聴するには、JoVE 購読が必要です。 サインイン又は無料トライアルを申し込む。
Generation of Organotypic Raft Cultures from Primary Human Keratinocytes
18,932 Views
•
07:26 min
•
February 22, 2012
DOI:
概要
Automatically generated
An in vitro method to mimic in vivo epithelial differentiation is described. Many viruses target epithelial cells as part of their viral life cycle, and this method provides a means of examining virus:host interactions that more closely resembles that which occurs in vivo. This technique can be used with primary keratinocytes, established cell lines, as well as normal or diseased biopsy tissue.
Explore More Videos
Organotypic Raft CulturesPrimary Human KeratinocytesIn Vitro SystemEpithelial DifferentiationThree-dimensional CulturesKeratinocytesHuman Papillomavirus (HPV)Virus ProductionDysplastic LesionsEpithelial Cell CancersDrugs On Epithelial Cell DifferentiationAsselineau And PrunierasKopan Et Al.Collagen PlugsAir-liquid InterfaceStratify And DifferentiateFull Thickness EpitheliumDifferentiation-specific CytokeratinsHistological ExaminationMolecular TechniquesBiochemical Techniques
Read Article
Cite this Article
Anacker, D., Moody, C. Generation of Organotypic Raft Cultures from Primary Human Keratinocytes. J. Vis. Exp. (60), e3668, doi:10.3791/3668 (2012).
Copy