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Immunology and Infection

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JoVE Journal Immunology and Infection
Generation of Organotypic Raft Cultures from Primary Human Keratinocytes
 

Generation of Organotypic Raft Cultures from Primary Human Keratinocytes

Article DOI: 10.3791/3668-v • 07:26 min • February 22nd, 2012
February 22nd, 2012 •
1, 1,2
1Department of Microbiology & Immunology, University of North Carolina-Chapel Hill, 2Lineberger Cancer Center, University of North Carolina-Chapel Hill

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An in vitro method to mimic in vivo epithelial differentiation is described. Many viruses target epithelial cells as part of their viral life cycle, and this method provides a means of examining virus:host interactions that more closely resembles that which occurs in vivo. This technique can be used with primary keratinocytes, established cell lines, as well as normal or diseased biopsy tissue.

Tags

Organotypic Raft Cultures Primary Human Keratinocytes In Vitro System Epithelial Differentiation Three-dimensional Cultures Keratinocytes Human Papillomavirus (HPV) Virus Production Dysplastic Lesions Epithelial Cell Cancers Drugs On Epithelial Cell Differentiation Asselineau And Prunieras Kopan Et Al. Collagen Plugs Air-liquid Interface Stratify And Differentiate Full Thickness Epithelium Differentiation-specific Cytokeratins Histological Examination Molecular Techniques Biochemical Techniques
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