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转基因小鼠内耳的发展<em>在体内</em>电穿孔
JoVE Journal
Neuroscience
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JoVE Journal Neuroscience
Gene Transfer to the Developing Mouse Inner Ear by In Vivo Electroporation
DOI:

22:02 min

June 30, 2012

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Chapters

  • 00:05Title
  • 01:58Part 1: Ventral Laparotomy
  • 05:23Ventral Midline Incision
  • 07:46Part 2: Transuterine Microinjection
  • 08:32Common Embryo Orientations Observed by Transillumination of the Uterus
  • 10:19Embryo Reorientation for Otocyst Injections
  • 13:48Transuterine Microinjection
  • 16:31Part 3: In vivo Electroporation
  • 17:37Postnatal Selection of a Pup whose Otocyst was Injected at E12.5
  • 19:47Prophylactic Analgesia
  • 20:18Surgical Subject the Morning after Surgery
  • 20:55Part 4: Representative Results

Summary

Automatic Translation

在小鼠内耳是一个基板派生的感觉器官,在妊娠期间阐述其发展计划。我们定义一个<em>在子宫内</em>转基因技术包括三个步骤:鼠标腹剖腹,transuterine显微注射,<em>在体内</em>电。我们用数字视频显微镜演示实验胚胎学技术关键。

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