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Gene Transfer to the Developing Mouse Inner Ear by In Vivo Electroporation
JoVE Journal
Neurowissenschaften
Author Produced
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JoVE Journal Neurowissenschaften
Gene Transfer to the Developing Mouse Inner Ear by In Vivo Electroporation

Gene Transfer to the Developing Mouse Inner Ear by In Vivo Electroporation

13,847 Views

22:02 min

June 30, 2012

DOI:

22:02 min
June 30, 2012

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Summary

Automatically generated

The mouse inner ear is a placode-derived sensory organ whose developmental program is elaborated during gestation. We define an in utero gene transfer technique consisting of three steps: mouse ventral laparotomy, transuterine microinjection, and in vivo electroporation. We use digital video microscopy to demonstrate the critical experimental embryological techniques.

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