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Quantifying the Activity of cis-Regulatory Elements in the Mouse Retina by Explant Electroporation
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June 28, 2011
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This protocol describes a simple and inexpensive way to quantify the activity of cis-regulatory elements (i.e., enhancer/promoters) in living mouse retinas via explant electroporation. DNA preparation, retinal dissection, electroporation, retinal explant culture, and post-fixation analysis and quantification are described.
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Montana, C. L., Myers, C. A., Corbo, J. C. Quantifying the Activity of cis-Regulatory Elements in the Mouse Retina by Explant Electroporation. J. Vis. Exp. (52), e2821, doi:10.3791/2821 (2011).
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