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浦肯野细胞树突形态的器官切片培养分析
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Neuroscience
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JoVE Journal Neuroscience
The Analysis of Purkinje Cell Dendritic Morphology in Organotypic Slice Cultures
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浦肯野细胞树突形态的器官切片培养分析

DOI:
10.3791/3637-v

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07:59 min

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March 21, 2012

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,
1Anatomical Institute, Department of Biomedicine,University of Basel

Chapters

  • 00:05Title
  • 01:48Setting up Organotypic Cerebellar Slice Cultures
  • 03:02Fixation and Immunostaining of Organotypic Cerebellar Slice Cultures
  • 04:52Measurement of Purkinje Cell Dendritic Tree Size and Branch Points
  • 06:07Representative Results for the Purkinje Cell Dendritic Tree Size and Branching
  • 07:23Conclusion

Summary

Automatic Translation

Automatic Translation

我们提出了一个协议，允许查看和定量驴个别浦肯野细胞的树突树的生长在器官小脑切片文化形态。该协议的目的是促进研究的浦肯野细胞树突发育的机制。

Tags

Purkinje CellDendritic MorphologyOrganotypic Slice CulturesSagittal PlanePostnatal PeriodAnti-Calbindin D28KEGFPJackson LabsCerebellar Slice CulturesDendritic DevelopmentDendritic ExpansionQuantitative EvaluationDendritic Tree SizeBranch Point NumbersAnti-calbindin StainedProtein Kinase C (PKC) Acti

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