/
/
The Analysis of Purkinje Cell Dendritic Morphology in Organotypic Slice Cultures
Un abonnement à JoVE est nécessaire pour voir ce contenu. Connectez-vous ou commencez votre essai gratuit.
The Analysis of Purkinje Cell Dendritic Morphology in Organotypic Slice Cultures
18,336 Views
•
07:59 min
•
March 21, 2012
DOI:
Summary
Automatically generated
We present a protocol that permits to view and to quantitatively asses the morphology of the dendritic tree of individual Purkinje cells grown in organotypic cerebellar slice cultures. This protocol is intended to promote studies on the mechanisms of Purkinje cell dendritic development.
Explore More Videos
Purkinje CellDendritic MorphologyOrganotypic Slice CulturesSagittal PlanePostnatal PeriodAnti-Calbindin D28KEGFPJackson LabsCerebellar Slice CulturesDendritic DevelopmentDendritic ExpansionQuantitative EvaluationDendritic Tree SizeBranch Point NumbersAnti-calbindin StainedProtein Kinase C (PKC) Acti
Read Article
Cite this Article
Kapfhammer, J. P., Gugger, O. S. The Analysis of Purkinje Cell Dendritic Morphology in Organotypic Slice Cultures. J. Vis. Exp. (61), e3637, doi:10.3791/3637 (2012).
Copy