ジャーナル
/
神経科学
/
Isolation and Expansion of Human Glioblastoma Multiforme Tumor Cells Using the Neurosphere Assay
JoVE Journal
神経科学
Author Produced
This content is Free Access.
JoVE Journal 神経科学
Isolation and Expansion of Human Glioblastoma Multiforme Tumor Cells Using the Neurosphere Assay

Isolation and Expansion of Human Glioblastoma Multiforme Tumor Cells Using the Neurosphere Assay

29,368 Views

•

09:24 min

•

October 30, 2011

DOI:

10.3791/3633-v

DOI:
10.3791/3633-v

•

09:24 min
October 30, 2011

•

9 Views
, , , , ,
1Department of Neurosurgery,University of Florida , 2Department of Anatomical Sciences,Shiraz University of Medical Sciences

概要

Automatically generated

This video protocol demonstrates the isolation and expansion of stem like cells from surgically resected human glioblastoma mutliforme (GBM) tumor tissue using the neurosphere assay culture method.

Explore More Videos

IsolationExpansionHuman Glioblastoma MultiformeTumor CellsNeurosphere AssayStem-like CellsBreastLungColonProstateBrainTumor Initiating Cell Population(s)Tumor FormationProgressionRecurrenceTherapeutic ApproachesNeurosphere Culture MethodChemical DigestionMechanical DissociationSingle Cell SuspensionNSA CulturePrimary Neurospheres

Read Article

Cite this Article

Azari, H., Millette, S., Ansari, S., Rahman, M., Deleyrolle, L. P., Reynolds, B. A. Isolation and Expansion of Human Glioblastoma Multiforme Tumor Cells Using the Neurosphere Assay. J. Vis. Exp. (56), e3633, doi:10.3791/3633 (2011).

Download .ris file

Copy

Share Video

.

Copy