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Encapsulated Hücre Agregalar içinde Takip Hipoksik Sinyalizasyonu
JoVE Journal
Bioengineering
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JoVE Journal Bioengineering
Tracking Hypoxic Signaling within Encapsulated Cell Aggregates
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Encapsulated Hücre Agregalar içinde Takip Hipoksik Sinyalizasyonu

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10,435 Views

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09:14 min

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December 16, 2011

DOI:

10.3791/3521-v

DOI:
10.3791/3521-v

•

09:14 min
December 16, 2011

•

2 Views
, ,
1Biomedical Engineering Program,University of South Carolina, 2Chemical Engineering Department,University of South Carolina

Summary

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Bir çapraz PEG hidrojel hücrelerin fotoğraf kapsülleme için bir yöntem açıklanmıştır. Kapsüllü fare İnsulinoma (MIN6) agrega içinde Hipoksik sinyal floresan marker sistemi kullanılarak izlenir. Bu sistem değişiklikleri ile hücre fenotipi hipoksik sinyal hidrojel iskele ve korelasyon içinde hücre seri muayene sağlar.

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Cite this Article

Skiles, M. L., Sahai, S., Blanchette, J. O. Tracking Hypoxic Signaling within Encapsulated Cell Aggregates. J. Vis. Exp. (58), e3521, doi:10.3791/3521 (2011).

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